貨號:25234 e-MycoTM Plus Mycoplasma PCR Detection Kit (over 8 genus 209 species ) 時間: 2008-12-10 13:05:11
eMyco™ plus Mycoplasma PCR Detection Kit greatly simplify testing and detection
of Mycoplasma contamination in cell cultures. With PCR testing, reliable results are
obtained within a few hours, since the presence of contaminant Mycoplasmas can be
easily and sensitively detected by simply verifying the bands of amplified DNA
fragments after gel electrophoresis. The eMyco™ plus Mycoplasma PCR Detection
has been shown to be a highly sensitive, specific and rapid method for the detection
of Mycoplasmas contamination in cell cultures.
Though the gene sequences for 16S rRNA are very similar in most Mycoplasma
species, there are some differences in the sequences of 16S rRNA gene between
certain Mycoplasma species and the other species.
Specific primers set of eMyco™ plus Mycoplasma PCR Detection Kit was designed
from DNA sequences that are coding for highly conserved 16S rRNA with
considering above point. Thus eMyco™ plus Mycoplasma PCR Detection Kit can be
used in the detection of more broad range of Mycoplasma species, compared with
any other commercially available PCRbased Mycoplasma detection kit, without
interfering with animal or bacterial DNA.
The internal control was designed in such a way that the primers set
was used to amplify the internal control and target DNA, which were differentiated by
size. Furthermore, the sample control was provided with this kit for using in verifying
the effectiveness of template DNA. So, You may easily check your sample
preparation. In addition, the use of 8methoxypsoralen (8MOP) was adopted in this
kit. 8MOP is known to intercalate into doublestranded nucleic acids and form a
covalent interstrand crosslink after photoactivation by incident light at wavelength
320400 nm, so it is helpful to prevent crosscontamination by PCR products from
earlier experiments.
Each tube of the eMyco™ plus Mycoplasma PCR Detection Kit contains all the
components for PCR except for template: DNA Polymerase, dNTPs, PCR Buffer,
primers set, 8MOP, and internal control. So, you can just add your templates and
perform the PCR reaction.